The development of the Pseudomonas aeruginosa outer membrane protein OprF as a presentation vector for foreign antigenic determinants

The development of the Pseudomonas aeruginosa outer membrane protein OprF as a presentation vector for foreign antigenic determinants

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A variety of systems have been developed to improve the presentation of foreign antigenic determinants (‘epitopes’) by inserting them in the context of carrier proteins. The goals of this study were to develop the Pseudomonas aeruginosa outer membrane protein OprF as a carrier for foreign epitopes a...

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Main Author: Wong, Rebecca Suk Yi
Format: Others
Language:English
Published: 2009
Online Access:http://hdl.handle.net/2429/7463
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spelling ndltd-UBC-oai-circle.library.ubc.ca-2429-74632018-01-05T17:33:43Z The development of the Pseudomonas aeruginosa outer membrane protein OprF as a presentation vector for foreign antigenic determinants Wong, Rebecca Suk Yi A variety of systems have been developed to improve the presentation of foreign antigenic determinants (‘epitopes’) by inserting them in the context of carrier proteins. The goals of this study were to develop the Pseudomonas aeruginosa outer membrane protein OprF as a carrier for foreign epitopes and to study the effect of the mode of presentation on the antigenicity of the presented epitope. The model epitope used in this study was the 4-amino acid repeating epitope (NANP) of the circumsporozoite protein of the malaria parasite, Plasmodium falciparum. Linker-insertion mutagenesis was carried out to create 11 “permissive” sites which allowed the insertion of 4 extra amino acids. Two series of OprF Science, Faculty of Microbiology and Immunology, Department of Graduate 2009-04-22T17:17:14Z 2009-04-22T17:17:14Z 1995 1995-05 Text Thesis/Dissertation http://hdl.handle.net/2429/7463 eng For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use. 3981613 bytes application/pdf
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language English
format Others
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description A variety of systems have been developed to improve the presentation of foreign antigenic determinants (‘epitopes’) by inserting them in the context of carrier proteins. The goals of this study were to develop the Pseudomonas aeruginosa outer membrane protein OprF as a carrier for foreign epitopes and to study the effect of the mode of presentation on the antigenicity of the presented epitope. The model epitope used in this study was the 4-amino acid repeating epitope (NANP) of the circumsporozoite protein of the malaria parasite, Plasmodium falciparum. Linker-insertion mutagenesis was carried out to create 11 “permissive” sites which allowed the insertion of 4 extra amino acids. Two series of OprF === Science, Faculty of === Microbiology and Immunology, Department of === Graduate
author Wong, Rebecca Suk Yi
spellingShingle Wong, Rebecca Suk Yi
The development of the Pseudomonas aeruginosa outer membrane protein OprF as a presentation vector for foreign antigenic determinants
author_facet Wong, Rebecca Suk Yi
author_sort Wong, Rebecca Suk Yi
title The development of the Pseudomonas aeruginosa outer membrane protein OprF as a presentation vector for foreign antigenic determinants
title_short The development of the Pseudomonas aeruginosa outer membrane protein OprF as a presentation vector for foreign antigenic determinants
title_full The development of the Pseudomonas aeruginosa outer membrane protein OprF as a presentation vector for foreign antigenic determinants
title_fullStr The development of the Pseudomonas aeruginosa outer membrane protein OprF as a presentation vector for foreign antigenic determinants
title_full_unstemmed The development of the Pseudomonas aeruginosa outer membrane protein OprF as a presentation vector for foreign antigenic determinants
title_sort development of the pseudomonas aeruginosa outer membrane protein oprf as a presentation vector for foreign antigenic determinants
publishDate 2009
url http://hdl.handle.net/2429/7463
work_keys_str_mv AT wongrebeccasukyi thedevelopmentofthepseudomonasaeruginosaoutermembraneproteinoprfasapresentationvectorforforeignantigenicdeterminants
AT wongrebeccasukyi developmentofthepseudomonasaeruginosaoutermembraneproteinoprfasapresentationvectorforforeignantigenicdeterminants
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