The development of the Pseudomonas aeruginosa outer membrane protein OprF as a presentation vector for foreign antigenic determinants
A variety of systems have been developed to improve the presentation of foreign antigenic determinants (‘epitopes’) by inserting them in the context of carrier proteins. The goals of this study were to develop the Pseudomonas aeruginosa outer membrane protein OprF as a carrier for foreign epitopes a...
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| Format: | Others |
| Language: | English |
| Published: |
2009
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| Online Access: | http://hdl.handle.net/2429/7463 |
| Summary: | A variety of systems have been developed to improve the presentation of
foreign antigenic determinants (‘epitopes’) by inserting them in the context of
carrier proteins. The goals of this study were to develop the Pseudomonas
aeruginosa outer membrane protein OprF as a carrier for foreign epitopes and to
study the effect of the mode of presentation on the antigenicity of the presented
epitope. The model epitope used in this study was the 4-amino acid repeating
epitope (NANP) of the circumsporozoite protein of the malaria parasite,
Plasmodium falciparum. Linker-insertion mutagenesis was carried out to create
11 “permissive” sites which allowed the insertion of 4 extra amino acids. Two series
of OprF === Science, Faculty of === Microbiology and Immunology, Department of === Graduate |
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