Molecular cloning of blueberry shock ilarvirus (BSIV) RNA 3 containing the coat protein gene and identification of a virus associated with BSIV-infected blueberry blossoms
Blueberry shock ilarvirus (BSIV) was cloned using random primers. Complementary DNA (cDNA) was ligated into Bluescript II and used to transform into DH5a Escherichia coil. The plasmids were screened for BSIV RNA 3 cDNA by Northern, Southern and dot blots. Seven clones were restriction mapped. A 4...
| Main Author: | |
|---|---|
| Format: | Others |
| Language: | English |
| Published: |
2009
|
| Online Access: | http://hdl.handle.net/2429/5196 |