DNA:RNA hybrid genome-wide profiling and links to genomic instability

DNA:RNA hybrid formation is emerging as a significant cause of genomic instability in biological systems ranging from bacteria to mammals. However, the scope of cellular pathways that prevent DNA:RNA hybrids and the genomic loci prone to hybrid formation are unclear. In Saccharomyces cerevisiae, DNA...

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Main Author: Chan, Yujia Alina
Language:English
Published: University of British Columbia 2014
Online Access:http://hdl.handle.net/2429/46327
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spelling ndltd-UBC-oai-circle.library.ubc.ca-2429-463272018-01-05T17:27:15Z DNA:RNA hybrid genome-wide profiling and links to genomic instability Chan, Yujia Alina DNA:RNA hybrid formation is emerging as a significant cause of genomic instability in biological systems ranging from bacteria to mammals. However, the scope of cellular pathways that prevent DNA:RNA hybrids and the genomic loci prone to hybrid formation are unclear. In Saccharomyces cerevisiae, DNA:RNA hybrids were found to be prevalent in various RNA processing, DNA repair and kinetochore mutants. In particular, mRNA cleavage and polyadenylation factors were demonstrated to maintain genome integrity by preventing transcription-dependent DNA:RNA hybrid formation. Genome-wide profiling of DNA:RNA hybrids showed that highly transcribed genes are prone to hybrid formation in the absence of hybrid-mitigating enzymes. Furthermore, the hybrid profiles highlight various genetic features prone to hybrid formation and suggest potential functions for DNA:RNA hybrids in antisense transcription regulation. Together, these findings elucidate previously unrecognized pathways that mitigate DNA:RNA hybrid formation as well as the characteristics of hybrid prone genomic regions. Medicine, Faculty of Biochemistry and Molecular Biology, Department of Graduate 2014-04-07T14:26:19Z 2014-10-31T00:00:00Z 2014 2014-05 Text Thesis/Dissertation http://hdl.handle.net/2429/46327 eng Attribution-NonCommercial-NoDerivs 2.5 Canada http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ University of British Columbia
collection NDLTD
language English
sources NDLTD
description DNA:RNA hybrid formation is emerging as a significant cause of genomic instability in biological systems ranging from bacteria to mammals. However, the scope of cellular pathways that prevent DNA:RNA hybrids and the genomic loci prone to hybrid formation are unclear. In Saccharomyces cerevisiae, DNA:RNA hybrids were found to be prevalent in various RNA processing, DNA repair and kinetochore mutants. In particular, mRNA cleavage and polyadenylation factors were demonstrated to maintain genome integrity by preventing transcription-dependent DNA:RNA hybrid formation. Genome-wide profiling of DNA:RNA hybrids showed that highly transcribed genes are prone to hybrid formation in the absence of hybrid-mitigating enzymes. Furthermore, the hybrid profiles highlight various genetic features prone to hybrid formation and suggest potential functions for DNA:RNA hybrids in antisense transcription regulation. Together, these findings elucidate previously unrecognized pathways that mitigate DNA:RNA hybrid formation as well as the characteristics of hybrid prone genomic regions. === Medicine, Faculty of === Biochemistry and Molecular Biology, Department of === Graduate
author Chan, Yujia Alina
spellingShingle Chan, Yujia Alina
DNA:RNA hybrid genome-wide profiling and links to genomic instability
author_facet Chan, Yujia Alina
author_sort Chan, Yujia Alina
title DNA:RNA hybrid genome-wide profiling and links to genomic instability
title_short DNA:RNA hybrid genome-wide profiling and links to genomic instability
title_full DNA:RNA hybrid genome-wide profiling and links to genomic instability
title_fullStr DNA:RNA hybrid genome-wide profiling and links to genomic instability
title_full_unstemmed DNA:RNA hybrid genome-wide profiling and links to genomic instability
title_sort dna:rna hybrid genome-wide profiling and links to genomic instability
publisher University of British Columbia
publishDate 2014
url http://hdl.handle.net/2429/46327
work_keys_str_mv AT chanyujiaalina dnarnahybridgenomewideprofilingandlinkstogenomicinstability
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