| Summary: | Cre recombinase of bacteriophage PI has become a useful tool for molecular
biological studies in vivo as well as in vitro. The Cre protein is an essential part of a
system which catalyzes recombination between specific DNA sequence repeats called loxP
sites. This recombination system allows for excision or inversion, depending on loxP
orientation, Of DNA sequences flanked by loxP sites. In order to utilize the recombinase in
mammalian systems, the retroviral vectors, LXSN and LXSHD, have been incorporated
into the production of shuttle vectors and retroviral producing cell lines. The produced
retroviruses allow for the transfer into and expression of the Cre recombinase in
mammalian cells. These newly constructed retroviral particles will be a useful tool for
inducing specific gene ablation in infective mammalian cells. === Medicine, Faculty of === Medical Genetics, Department of === Graduate
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