The isolation and phenotypic characterization of Paramecium tetraurelia temperature-sensitive cell cycle mutants

Conditional cell cycle mutants which block cell division under restrictive conditions, increase their cell mass as a result of continued growth. Here, we develop a protocol to screen for large Paramecium temperature-sensitive cell cycle mutants by sizeselecting centrifugal elutriation. By using t...

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Bibliographic Details
Main Author: Chua, Gordon
Format: Others
Language:English
Published: 2009
Online Access:http://hdl.handle.net/2429/3522

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