Characterization of a human prothrombin gene enhancer

The 5' flanking sequence of the human prothrombin gene was isolated by screening a human liver phage library with a human prothrombin cDNA as a hybridization probe. A phage was identified that contained 3 kilobasepairs of DNA upstream of the initiator methionine codon. Primer extension studies...

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Main Author: Chow, Billy Kowk-Chong
Language:English
Published: University of British Columbia 2011
Online Access:http://hdl.handle.net/2429/30989
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spelling ndltd-UBC-oai-circle.library.ubc.ca-2429-309892018-01-05T17:45:49Z Characterization of a human prothrombin gene enhancer Chow, Billy Kowk-Chong The 5' flanking sequence of the human prothrombin gene was isolated by screening a human liver phage library with a human prothrombin cDNA as a hybridization probe. A phage was identified that contained 3 kilobasepairs of DNA upstream of the initiator methionine codon. Primer extension studies showed that the major transcription initiation sites were located 23 and 36 basepairs upstream of the initiator codon. DNA sequences in the 5' flanking region of the human prothrombin gene were then analyzed for cis-activating transcriptional activity by a transient expression system using the human growth hormone gene as the reporter gene. The chimeric expression vector was introduced into HepG2 cells, and secreted human growth hormone was monitored by using a radioimmunoassay. These studies showed that the 3 kbp fragment contained sequences that were sufficient for the initiation of transcription in HepG2 cells. Subsequent deletion studies showed that the 3 kbp fragment contained two elements: a weak promoter in the region immediately upstream of the mRNA coding sequence, and an enhancer located between nucleotides -860 and -940. The enhancer element was active at a distance and in either orientation. In addition, the enhancer was liver cell specific, and acted on heterologous promoters including the herpes simplex virus thymidine kinase promoter and the mouse metallothionein I promoter. Comparison of the nucleotide sequence of the enhancer with a DNA sequence data base showed the enhancer sequence to be unique. The enhancer sequence is flanked by an inverted repeat, 5' CCTCCC 3', and contains a putative binding site for hepatic nuclear factor 1 (HNF-1). Deoxyribonuclease I footprint analysis and linker scanning mutagenesis showed that the enhancer contains multiple protein binding motifs. Mutagenesis of the 3' boundary CCTCCC sequence eliminated the enhancer activity. Comparison with other liver genes showed the presence of the CCTCCC sequence in the hepatitis B virus enhancer, the ⍺-1 antitrypsin promoter, and the fibrinogen (β-chain promoter, suggesting a functional role for this motif. Using the concatenated human prothrombin enhancer as a probe to screen a HepG2 expression library, a cDNA encoding for the Y-box binding protein was identified. A putative Y-box was also found in the enhancer region, suggesting that the protein factor may be partially responsible for the human prothrombin gene expression. Northern blot analysis using the Y-box binding protein cDNA as a probe indicated that the Y-box binding protein mRNA is expressed in all of the tested tissues. This protein may be one of the constitutively expressed transcription factors responsible for the regulation of a number of genes. Medicine, Faculty of Biochemistry and Molecular Biology, Department of Graduate 2011-01-31T20:40:33Z 2011-01-31T20:40:33Z 1991 Text Thesis/Dissertation http://hdl.handle.net/2429/30989 eng For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use. University of British Columbia
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language English
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description The 5' flanking sequence of the human prothrombin gene was isolated by screening a human liver phage library with a human prothrombin cDNA as a hybridization probe. A phage was identified that contained 3 kilobasepairs of DNA upstream of the initiator methionine codon. Primer extension studies showed that the major transcription initiation sites were located 23 and 36 basepairs upstream of the initiator codon. DNA sequences in the 5' flanking region of the human prothrombin gene were then analyzed for cis-activating transcriptional activity by a transient expression system using the human growth hormone gene as the reporter gene. The chimeric expression vector was introduced into HepG2 cells, and secreted human growth hormone was monitored by using a radioimmunoassay. These studies showed that the 3 kbp fragment contained sequences that were sufficient for the initiation of transcription in HepG2 cells. Subsequent deletion studies showed that the 3 kbp fragment contained two elements: a weak promoter in the region immediately upstream of the mRNA coding sequence, and an enhancer located between nucleotides -860 and -940. The enhancer element was active at a distance and in either orientation. In addition, the enhancer was liver cell specific, and acted on heterologous promoters including the herpes simplex virus thymidine kinase promoter and the mouse metallothionein I promoter. Comparison of the nucleotide sequence of the enhancer with a DNA sequence data base showed the enhancer sequence to be unique. The enhancer sequence is flanked by an inverted repeat, 5' CCTCCC 3', and contains a putative binding site for hepatic nuclear factor 1 (HNF-1). Deoxyribonuclease I footprint analysis and linker scanning mutagenesis showed that the enhancer contains multiple protein binding motifs. Mutagenesis of the 3' boundary CCTCCC sequence eliminated the enhancer activity. Comparison with other liver genes showed the presence of the CCTCCC sequence in the hepatitis B virus enhancer, the ⍺-1 antitrypsin promoter, and the fibrinogen (β-chain promoter, suggesting a functional role for this motif. Using the concatenated human prothrombin enhancer as a probe to screen a HepG2 expression library, a cDNA encoding for the Y-box binding protein was identified. A putative Y-box was also found in the enhancer region, suggesting that the protein factor may be partially responsible for the human prothrombin gene expression. Northern blot analysis using the Y-box binding protein cDNA as a probe indicated that the Y-box binding protein mRNA is expressed in all of the tested tissues. This protein may be one of the constitutively expressed transcription factors responsible for the regulation of a number of genes. === Medicine, Faculty of === Biochemistry and Molecular Biology, Department of === Graduate
author Chow, Billy Kowk-Chong
spellingShingle Chow, Billy Kowk-Chong
Characterization of a human prothrombin gene enhancer
author_facet Chow, Billy Kowk-Chong
author_sort Chow, Billy Kowk-Chong
title Characterization of a human prothrombin gene enhancer
title_short Characterization of a human prothrombin gene enhancer
title_full Characterization of a human prothrombin gene enhancer
title_fullStr Characterization of a human prothrombin gene enhancer
title_full_unstemmed Characterization of a human prothrombin gene enhancer
title_sort characterization of a human prothrombin gene enhancer
publisher University of British Columbia
publishDate 2011
url http://hdl.handle.net/2429/30989
work_keys_str_mv AT chowbillykowkchong characterizationofahumanprothrombingeneenhancer
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