Topological analysis of the transhydrogenase in Escherichia coli membranes using proteolytic probes

Using proteolytic probes, the pyridine nucleotide transhydrogenase (EC 1.6.1.1) from Escherichia coli was analyzed for its native topography in the cytoplasmic membrane. Before analyses could be performed, the isolation of transhydrogenase-enriched ISO (inside-out) cytoplasmic membrane vesicles was...

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Bibliographic Details
Main Author: Tong, Raymond Cheuk Wa
Language:English
Published: University of British Columbia 2010
Subjects:
Online Access:http://hdl.handle.net/2429/30401

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