Recovery from exhaustive exercise in rainbow trout white muscle : a model for studies of the control of energy metabolism in vivo

Recovery from exhaustive exercise in the white muscle of rainbow trout (Oncorhynchus mykiss) was used to examine the role of the adenylates in the control of energy metabolism and to assess the validity of equilibrium models of the behaviour of the high energy phosphates. The difficulty of obtainin...

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Main Author: Schulte, Patricia Marita
Language:English
Published: University of British Columbia 2010
Subjects:
Online Access:http://hdl.handle.net/2429/29874
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spelling ndltd-UBC-oai-circle.library.ubc.ca-2429-298742018-01-05T17:45:23Z Recovery from exhaustive exercise in rainbow trout white muscle : a model for studies of the control of energy metabolism in vivo Schulte, Patricia Marita Rainbow trout -- Metabolism Oncorhynchus mykiss -- metabolism Energy metabolism -- Research -- Methodology Energy Metabolism Exercise -- Physiological aspects Exercise -- physiology Muscles -- Metabolism Recovery from exhaustive exercise in the white muscle of rainbow trout (Oncorhynchus mykiss) was used to examine the role of the adenylates in the control of energy metabolism and to assess the validity of equilibrium models of the behaviour of the high energy phosphates. The difficulty of obtaining muscle samples from fish makes detailed analysis of the behaviour of the labile high energy phosphates complex. The use of a new sampling procedure, the infusion of a lethal dose of anaesthetic via an indwelling cannula, minimized this problem. At exhaustion [ATP] and [PCr] were depressed by 75 and 80% respectively relative to the resting values. [ATP] depletion was mirrored by a stoichiometric increase in [IMP]. During recovery [PCr] returned to the resting level within 2 hours, but [ATP] recovery was slow and not complete until 24 hours post exercise. In contrast, energy charge and RATP(the proportion of the free adenylate pool phosphorylated to ATP) were, if anything, higher than the resting values by 2 hours post exercise. Therefore, [ATP] and energy status can be dissociated in tissues like fish white muscle because of the action of the purine nucleotide cycle. At 2 hours post exercise the calculated free ADP concentration dropped to less than one tenth the value at rest. As a result the [ATP] / [ADP] free ratio increased by nearly 6 fold. This condition may be required for glycogen resynthesis from lactate in muscle. Several similar equilibrium models of the behaviour of the adenylates and PCr were applied to the fish white muscle system. In general, the models well describe the relationship between the high energy phosphates. However, the definition of the high energy phosphate pool introduces some complications since this includes the total [ATP]. Because of the action of AMP deaminase the [ATP] concentration can change without measurable changes in the energy status, which is not considered in any of the models. As long as the extent of IMP formation is known the models can be applied, but since the formation of IMP may vary from fish to fish or with exercise regime the models lose much of their predictive power. Science, Faculty of Zoology, Department of Graduate 2010-11-08T21:26:18Z 2010-11-08T21:26:18Z 1990 Text Thesis/Dissertation http://hdl.handle.net/2429/29874 eng For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use. University of British Columbia
collection NDLTD
language English
sources NDLTD
topic Rainbow trout -- Metabolism
Oncorhynchus mykiss -- metabolism
Energy metabolism -- Research -- Methodology
Energy Metabolism
Exercise -- Physiological aspects
Exercise -- physiology
Muscles -- Metabolism
spellingShingle Rainbow trout -- Metabolism
Oncorhynchus mykiss -- metabolism
Energy metabolism -- Research -- Methodology
Energy Metabolism
Exercise -- Physiological aspects
Exercise -- physiology
Muscles -- Metabolism
Schulte, Patricia Marita
Recovery from exhaustive exercise in rainbow trout white muscle : a model for studies of the control of energy metabolism in vivo
description Recovery from exhaustive exercise in the white muscle of rainbow trout (Oncorhynchus mykiss) was used to examine the role of the adenylates in the control of energy metabolism and to assess the validity of equilibrium models of the behaviour of the high energy phosphates. The difficulty of obtaining muscle samples from fish makes detailed analysis of the behaviour of the labile high energy phosphates complex. The use of a new sampling procedure, the infusion of a lethal dose of anaesthetic via an indwelling cannula, minimized this problem. At exhaustion [ATP] and [PCr] were depressed by 75 and 80% respectively relative to the resting values. [ATP] depletion was mirrored by a stoichiometric increase in [IMP]. During recovery [PCr] returned to the resting level within 2 hours, but [ATP] recovery was slow and not complete until 24 hours post exercise. In contrast, energy charge and RATP(the proportion of the free adenylate pool phosphorylated to ATP) were, if anything, higher than the resting values by 2 hours post exercise. Therefore, [ATP] and energy status can be dissociated in tissues like fish white muscle because of the action of the purine nucleotide cycle. At 2 hours post exercise the calculated free ADP concentration dropped to less than one tenth the value at rest. As a result the [ATP] / [ADP] free ratio increased by nearly 6 fold. This condition may be required for glycogen resynthesis from lactate in muscle. Several similar equilibrium models of the behaviour of the adenylates and PCr were applied to the fish white muscle system. In general, the models well describe the relationship between the high energy phosphates. However, the definition of the high energy phosphate pool introduces some complications since this includes the total [ATP]. Because of the action of AMP deaminase the [ATP] concentration can change without measurable changes in the energy status, which is not considered in any of the models. As long as the extent of IMP formation is known the models can be applied, but since the formation of IMP may vary from fish to fish or with exercise regime the models lose much of their predictive power. === Science, Faculty of === Zoology, Department of === Graduate
author Schulte, Patricia Marita
author_facet Schulte, Patricia Marita
author_sort Schulte, Patricia Marita
title Recovery from exhaustive exercise in rainbow trout white muscle : a model for studies of the control of energy metabolism in vivo
title_short Recovery from exhaustive exercise in rainbow trout white muscle : a model for studies of the control of energy metabolism in vivo
title_full Recovery from exhaustive exercise in rainbow trout white muscle : a model for studies of the control of energy metabolism in vivo
title_fullStr Recovery from exhaustive exercise in rainbow trout white muscle : a model for studies of the control of energy metabolism in vivo
title_full_unstemmed Recovery from exhaustive exercise in rainbow trout white muscle : a model for studies of the control of energy metabolism in vivo
title_sort recovery from exhaustive exercise in rainbow trout white muscle : a model for studies of the control of energy metabolism in vivo
publisher University of British Columbia
publishDate 2010
url http://hdl.handle.net/2429/29874
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