The construction and characterization of a Pro-Thr box deletion of a Cellulomonas fimi endoglucanase (Cen A)

The catalytic domain is separated from the cellulose-binding domain in Cellulomonas fimi endoglucanase CenA by a proline-threonine rich sequence called the Pro-Thr box. To study the function of the Pro-Thr box region, a deletion mutant, cenAAPT, was made from cenA by an oligonucleotide directed in v...

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Bibliographic Details
Main Author: Shen, Hua
Language:English
Published: University of British Columbia 2010
Subjects:
Online Access:http://hdl.handle.net/2429/28744