A DNA-binding protein from Hela cells which binds preferentially to DNA damaged with ultraviolet light or N-acetoxy-N-acetyl-2-aminofluorene

A DNA-binding protein, PHI, was partially purified from extracts of Hela cells by high-speed centrifugation, and chromatography on DEAE-cellulose, phosphocellulose and UV-irradiated DNA-cellulose columns. It eluted from the phosphocellulose column with 0.375 M potassium phosphate and from the UV-irr...

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Bibliographic Details
Main Author: Tsang, Siu Sing
Language:English
Published: 2010
Online Access:http://hdl.handle.net/2429/22860