Isolation and characterization of restriction endonucleases from Fusobacterium nucleatum

• Main Author: Lui, Ann Chin Par
• Language: English
• Published: 2010
• Online Access: http://hdl.handle.net/2429/20826

Five strains of Fusobaotevium nucleatum were screened for the presence of restriction enzymes and the following recognition specificities and cleavage sites (indicated by the arrows ↓) were characterized: Fnu AI, 5' -G↓ANTC-3' ; Fnu CJ, 5' -↓GATC-3' (unmodified bases only); Fnu DI, 5'-GG↓CC-3'; Fnu DII , 5' -CG↓CG-3' ; Fnu DIII, 5'-GCG↓C-3'; Fnu EI, 5'- GATC-3' (both modified and unmodified bases recognized); Fnu 48I, 5'-TC↓GA-3'. === Medicine, Faculty of === Biochemistry and Molecular Biology, Department of === Graduate