Light and electron microscopic autoradiographic investigation of the septo-dentate pathway in rat brain

This study was undertaken to resolve the conflict between two existing studies regarding the layer in the dentate gyrus where the septal fibers terminate. This was accomplished by injecting radioact ively labelled amino acids into the medial septum where they were incorporated into protein by the...

Full description

Bibliographic Details
Main Author: Rose, Ann Marie
Language:English
Published: 2010
Subjects:
Online Access:http://hdl.handle.net/2429/19827
id ndltd-UBC-oai-circle.library.ubc.ca-2429-19827
record_format oai_dc
spelling ndltd-UBC-oai-circle.library.ubc.ca-2429-198272018-01-05T17:40:11Z Light and electron microscopic autoradiographic investigation of the septo-dentate pathway in rat brain Rose, Ann Marie Synapses Hippocampus (Brain) Hippocampus This study was undertaken to resolve the conflict between two existing studies regarding the layer in the dentate gyrus where the septal fibers terminate. This was accomplished by injecting radioact ively labelled amino acids into the medial septum where they were incorporated into protein by the cell bodies and transported to the nerve terminals. Autoradiographic grain counts showed labelling was moderately heavy in the subgranular zone of the dentate gyrus, whereas labelling was scant in the molecular layer. These findings support the work of Raisman et al., (1965), but indicate the degeneration seen by Mosko, et al., (1973) in the molecular layer may have been artefactual. The ultrastructure of the septal terminals in the subgranular zone of the dentate gyrus was described. These terminals formed asymmetrical synapses onto dendrites and spines, and contained clear round vesicles. They resembled cholinergic nerve terminals described in other regions of the brain. These findings agree with accumulating evidence that the septo-dentate pathway is cholinergic. Medicine, Faculty of Graduate 2010-02-08T22:08:02Z 2010-02-08T22:08:02Z 1976 Text Thesis/Dissertation http://hdl.handle.net/2429/19827 eng For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.
collection NDLTD
language English
sources NDLTD
topic Synapses
Hippocampus (Brain)
Hippocampus
spellingShingle Synapses
Hippocampus (Brain)
Hippocampus
Rose, Ann Marie
Light and electron microscopic autoradiographic investigation of the septo-dentate pathway in rat brain
description This study was undertaken to resolve the conflict between two existing studies regarding the layer in the dentate gyrus where the septal fibers terminate. This was accomplished by injecting radioact ively labelled amino acids into the medial septum where they were incorporated into protein by the cell bodies and transported to the nerve terminals. Autoradiographic grain counts showed labelling was moderately heavy in the subgranular zone of the dentate gyrus, whereas labelling was scant in the molecular layer. These findings support the work of Raisman et al., (1965), but indicate the degeneration seen by Mosko, et al., (1973) in the molecular layer may have been artefactual. The ultrastructure of the septal terminals in the subgranular zone of the dentate gyrus was described. These terminals formed asymmetrical synapses onto dendrites and spines, and contained clear round vesicles. They resembled cholinergic nerve terminals described in other regions of the brain. These findings agree with accumulating evidence that the septo-dentate pathway is cholinergic. === Medicine, Faculty of === Graduate
author Rose, Ann Marie
author_facet Rose, Ann Marie
author_sort Rose, Ann Marie
title Light and electron microscopic autoradiographic investigation of the septo-dentate pathway in rat brain
title_short Light and electron microscopic autoradiographic investigation of the septo-dentate pathway in rat brain
title_full Light and electron microscopic autoradiographic investigation of the septo-dentate pathway in rat brain
title_fullStr Light and electron microscopic autoradiographic investigation of the septo-dentate pathway in rat brain
title_full_unstemmed Light and electron microscopic autoradiographic investigation of the septo-dentate pathway in rat brain
title_sort light and electron microscopic autoradiographic investigation of the septo-dentate pathway in rat brain
publishDate 2010
url http://hdl.handle.net/2429/19827
work_keys_str_mv AT roseannmarie lightandelectronmicroscopicautoradiographicinvestigationoftheseptodentatepathwayinratbrain
_version_ 1718591248969760768