The effect of process conditions on productivity and glycosylation of cystatin C in P. pastoris and DNA microarray analysis of amino acid limitations in S. cerevisiae culture
Human cystatin C is a cysteine-proteinase inhibitor with several potential therapeutic applications. A recombinant variant of cystatin C with two potential sites for N-linked glycosylation (Nakamura, Ogawa et al. 2000) was selected for expression in the yeast Pichia pastoris. Glycosylation has been...
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| Format: | Others |
| Language: | English |
| Published: |
2009
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| Online Access: | http://hdl.handle.net/2429/14164 |