Characterization and modification of two carbohydrate-binding modules
The C-terminal carbohydrate-binding module of Xylanase 10A CBM2a, from Cellulomonas fimi was produced and secreted by the methylotrophic yeast Pichia pastoris. The polypeptide was highly N-glycosylated. MALDI-TOF mass spectrometry combined with protease digests and site-directed mutation located...
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| Format: | Others |
| Language: | English |
| Published: |
2009
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| Online Access: | http://hdl.handle.net/2429/11379 |