The role of CD164 in glioblastoma

• Main Authors: WANG, CHUNG-CHING, 王鐘慶
• Other Authors: CHAN, JAMES YI-HSIN; HUANG, SHIH-MING
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/vnchm9

博士 === 國防醫學院 === 醫學科學研究所 === 108 === Sialomucin clusters of differentiation 164 (CD164) was initially expressed because of its function on the surface of human CD34+ hematopoietic stem cells. CD164 have been proved to promote tumor formation, invasion and migration of lymphoma, prostate cancer, colon cancer, ovarian cancer, lung cancer and brain cancer. This dissertation found that tissue array analysis showed a positive correlation between expression level of CD164 and grade of glioma. In the PRECOG database, the expression of CD164 in astrocytoma/glioma patients had a significant negative correlation between CD164 expression and survival. Next, strategy was to inhibit the expression of CD164 molecules in brain cancer cell lines U87MG and U118MG. The results showed that the proliferation, migration and invasion of brain cancer cell lines were significantly decreased, and these effects were found to be associated with decreased Akt/mTOR signaling and increased autophagy. The application of one Akt inhibitor MK-2206 to mimic the changes in signal transduction after inhibition of CD164. The results showed that the increase in Beclin-1 and LC3B and the decrease in p62 were induced after treatment of MK-2206 and inhibition of CD164. According to this dissertation, CD164 is highly expressed in glioma cells by inhibiting autophagy and promoting tumor growth. Further experiments are needed to elucidate the role of CD164 in SDF-1ɑ/CXCR4 signaling in GBM cells, its regulation of autophagy and apoptosis, and its subsequent effects on tumor growth and survival.