Fabrication of epidermal growth factor-loaded core-shell gelatin nanofibers by coaxial electrospinning for skin regeneration

• Main Authors: CHEN, PEI-YIN, 陳姵吟
• Other Authors: CHEN, KUO-YU
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/d3peae

碩士 === 國立雲林科技大學 === 化學工程與材料工程系 === 107 === Nanofibrous membranes produced by electrospinning possess a large surface area-to-volume ratio, which mimic the three-dimensional structure of the extracellular matrix. Coaxial electrospinning can produce core-shell structure with two different layers. The shell layer serves as a barrier to prevent the premature release of the water-soluble core contents. In this study, gelatin electrospun nanofibers with core-shell structure were electrospun onto the chitosan membrane as scaffolding materials for skin regeneration. Epidermal growth factor (EGF) was incorporated into the core layer of the gelatin nanofibers to enhance wound healing. The morphology of nanofibers and their mean diameters were analyzed by a scanning electron microscopy. The core–shell structure of nanofibers was examined using a transmission electron microscopy. To determine the distribution of EGF within the core–shell nanofibers, the gelatin nanofibers with FITC-BSA were observed under the laser confocal scanning microscopy. The optimal electrospinning parameters were found as follow: applied voltage = 25 kV and tip-to-collector distance = 15 cm. The electrospun gelatin membranes were composed of uniform and beads-free nanofibers with an average diameter of 156.02 ± 69.36 nm. The transmission electron microscopy image illustrated the core–shell structure of the coaxial nanofibers. Moreover, the laser scanning confocal microscopy image indicated that FITC-BSA was distributed fairly evenly uniformly in the core layer of the gelatin nanofibers. The in vitro viability assay demonstrated that the incorporation of EGF could enhance fibroblast adhesion and proliferation. The animal experiment results indicated the EGF-loaded gelatin/chitosan bilayer membrane was very effective as a wound-healing accelerator in early-stage wound healing. Moreover, the skin wound treated with the EGF-loaded gelatin/chitosan bilayer membrane had more collagen deposition than that without EGF. Therefore, the EGF-loaded gelatin/chitosan bilayer membrane could be suggested potential for wound dressing applications.