Silk Fibroin in biomedical application: (1) properties of different strains of SF, (2) properties of SF/hyaluronic acid hydrogel and scaffold, (3) application in nasal drug delivery

• Main Authors: Ting-Ya Wu, 吳庭亞
• Other Authors: Tze-Wen Chung
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/46xb95

碩士 === 國立陽明大學 === 生物醫學工程學系 === 107 === Silk fibroin (SF) has good biocompatibility and mechanical strength, and has been widely used in the field of biomedicine. However, the different strains of silkworms for influence of their properties has not been explored. In this study, properties of these three strain of GF, MJ and ST will be investigated in the first part., and combined with hyaluronic acid (HA) to prepare hydrogel (HS gel) and scaffold and explore their difference properties for different applications in tissue engineering. Results of amino acid composition analysis showed that tyrosine amount in MJ-spring SF were the highest. Then the characteristic peaks and pore sizes of HS gel and scaffold were determined by ATR-FTIR spectra and SEM. The mechanical properties were analyzed by rheometer and compression test. The results show that the G' of MJ gel is increased from 3.24±0.16 kPa to 4.09±0.32 kPa compared with GF gel, and the compression modulus of scaffold is also increased from 0.95±0.08 MPa to 1.1±0.05 MPa, indicated that MJ, with higher tyrosine amount, resulted in higher crosslink degrees and further elevated the mechanical properties. In the second part, N-Acetyl-L-cysteine (NAC) -encapsulated nanoparticles loaded into the hydrogel can served as nose preparations. The nasal delivery to the brain can provide a more convenient drug administration across blood-brain barrier. After co-culture of SF/NAC NP with nasal epithelial cells (RPMI 2650), the nanoparticles can be observed through the transcellular pathway. The electrical resistance of epithelial cells measured by transepithelial electrical resistance (TEER) showed that SF/NAC NP and chitooligosaccharides (COS) can open tight junction in-between cells and improve the paracellular transport. NAC permeability toward RPMI 2650 cells showed that NAC, SF/NAC NP and SF/NAC NP/HS gel were 10.39±0.14×10-6 cm/s, 7.50±0.59×10-6 cm/s and 1.79±0.14×10-6 cm/s indicated that with the form as NP or gel can extend drug permeability and increase the bioavailability of the drug. This experiment analyzed different strains of SF to prepare HS gel and scaffold with different mechanical properties, which can be used as different tissue engineering and drug release applications. In addition, SF/NAC NP loaded into HS gel can make drugs through the transcellular (cell uptake) and the paracellular (tight junction) pathway for drug delivery as nasal preparations.