Detecting the level of nitrosylated Aβ: a potential biomarker for Alzheimer\'s disease

• Main Authors: Yun-Sheng Zhong, 鍾耘昇
• Other Authors: Nien-Jung Chen
• Format: Others
• Language: en_US
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/7ge6e5

碩士 === 國立陽明大學 === 微生物及免疫學研究所 === 107 === Alzheimer’s disease (AD) is the most common neurodegenerative disease. Previous researches indicated that there are many abnormal amyloid (Aβ) plaques deposited in AD patient’s brain, especially the hippocampus where is related to the memory functions. These Aβ proteins can aggregate into insoluble form and trigger inflammatory reaction, then cause the surrounding neuron cells death and result in irreversible damage. Many evidences indicated that early diagnosis is important for disease prevention and patient care plans. To investigate an efficient screening, cost-benefit early diagnostic method, we found that in previous report, the oxidase stress factor such as Nitric Oxide (NO) caused by Aβ deposition can induce Aβ nitrosylation on Tyrosine10, and Kummer et al. found that these nitrated Aβs only appeared in the core region of the plaque accumulated in AD’s brain. In this study, we firstly found that stimulating the BV2 cells with oligomer Aβ alone can enhance the Aβ nitrosylation, and the level of Aβ nitrosylation were increased while the cells were stimulated with oligomer Aβ and the NO donor. These results indicate that NO is important for Aβ protein nitrosylation. To further explore whether the nitrated Aβ might be a potential biomarker for dementia diagnosed, we establish two Nitrated Aβ detecting platform and using the case-control study to analysis the correlation between total Aβ/nitrated Aβ and disease progression. In our results, the risk that regarded as AD or MCI are increased when the serum level of total Aβ above a certain concentration. Moreover, the nitrated Aβ level are higher only in the samples from MCI patients, suggesting that nitrated Aβ might be a potential biomarker for the early dementia.