Acute anserine supplementation on oxidative stress andexercise performance

• Main Authors: Feng, Wen-Hsin, 豐文馨
• Other Authors: Hou, Chien-Wen
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/wmk788

碩士 === 臺北市立大學 === 運動科學研究所 === 107 === Aim : To investigate the effects of acute anserine supplementation on the performance of aerobic and anaerobic exercise as well as the antioxidative capacity in aerobic exercise. Methods: Twenty subjects were enrolled in this study. The subjects were randomly separated in two parts, Aerobic exercise (50% VO2 max) and Anaerobic exercise (60s Wingate) respectively. And both parts were crossover designed. Supplements were given one hour before exercise, which were low dose (15 mg/kg), high dose (30 mg/kg) and placebo (PLA), and washed out for 14 days. For aerobic exercise, we were collecting blood on the fasting state, 5 minutes after exercise, 60 minutes after exercise and 24 hours after exercise to measure oxidative stress and cell damage biomarker. The total running time was regarded as an aerobic performance indicator. We measured lactate on the fasting state, before exercise, 5 minutes and 15 minutes after anaerobic exercise, and the anaerobic performance indicator was the power output and fatigue index. Results: Among the oxidative stress biomarkers of aerobic exercise, high dose can significantly increase SOD compared with low dose and PLA (p < 0.001). Both low dose (p < 0.01) and high dose (p < 0.001) can significantly increase GSSG. TBARS, GSH and CAT were no difference between group. For the cell damage biomarkers, GPT was significantly higher in high dose than low dose (p < 0.05), but there was no difference compared with PLA. High dose showed higher CKMB than PLA (p < 0.05). GOT and myoglobin levels were no difference between group. Total exercise time was no difference iv between group. On the other hand, the mean power output in anaerobic exercise performance was enhanced in high dose compared with low dose and PLA (p < 0.05), but there was no effect on anaerobic peak power output and fatigue index. Blood lactate was increased by exercise in three group, but there was no difference between groups. Conclusion: Acute anserine supplementation can increase activities of SOD and GSSG during aerobic exercise and anaerobic mean power output. However, the biochemical mechanism to affect physiological functions of anserine need further investigation.