Study on Phage-containing Chitosan Film as Antibacterial Wound Dressing

• Main Authors: LIU, ZI-HAO, 劉子豪
• Other Authors: LIN, HSIN-YI
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/66x53g

碩士 === 國立臺北科技大學 === 化學工程與生物科技系化學工程碩士班 === 107 === In recent years, new bacteria increase rapidly, most bacteria have antibiotic resistance factors, lead to a reduction in available antibiotics. Antibiotic can't even inhibition or eliminate these bacteria. Therefore, phage can be used us a natural bacteriostatic material, and the phage is grafted onto the chitosan film to prepare a natural dressing having a bacteriostatic function. In this study, the Escherichia coli (Dh5α) was mixed with seawater for cultivation, and then the phage with inhibition of Escherichia coli was extracted, and the phage was grafted to the chitosan film by using glutaraldehyde to prepare a phage-containing chitosan film. In order to understand that the phage-containing chitosan film still has bacteriostatic activity and no cytotoxic, thus we measured number of grafts, bacteria culture on the film, cytotoxicity, and discussed whether the phage-containing chitosan film can be applied to skin dressing. The results of grafting show phage can indeed graft on the surface of chitosan film by glutaraldehyde, and the number of grafts reaches 1.52×105 PFU per film. The results of the bacteria culture experiment indicate that the phage-containing chitosan film after experimental processing still has the ability to inhibit bacteria. The results of cytotoxicity showed that both films had good cell activity, and that observed to have good adhesion and secretion behavior by electron microscopy, it indicates that the phage-containing chitosan film did not affect cell proliferation. Physics test results show that the phage-containing chitosan film has good mechanical strength, and the chitosan film does not degrade rapidly during treatment. In summary, in the era of antibiotics, a specific phage can be cultured from the environment, and combined with a chitosan film to produce a phage-containing dressing, which is a good inhibition method for drug-resistant bacteria of skin wounds. In the future, it is hoped that phage therapy can replace or assist antibiotic therapy and become a valuable antibacterial dressing.