Development of a Microfluidic Paper-based Analytical Device Based On Near-Infrared Emitting Iridium(III) Complex for Glucose Detection In Human Blood

• Main Authors: LIN, PENG-YI, 林芃儀
• Other Authors: Ho, MEI-LIN
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/9r4t3e

碩士 === 東吳大學 === 化學系 === 107 === The first part is "Development of a portable fluorescent detector", with the increasing number of aging population, our research target has become to develop a simple and a small equipment to achieve real-time monitoring health condition. This article uses 3D printing technology to create a portable fluorometer that can be combined with mobile phones. The portable fluorometer includes a first body part, a second body part and a support body part. The design with the advantages of small size, easy storage, less interference from the scattering light, and low-cast. An approved patent application has been provided by the Intellectual Property Office, and the patent certificate number is: M566327. The second part is "Design Satereogram", The main content is to draw, Three-dimensional figures by using the 3D software. A large amount of text information in books, newspapers and magazines often combines pictures to assist in explanations, increasing people's understanding of words and enhancing memory. Besides, with the advancement of technology, the development of computer graphics software has advanced from the basic Microsoft Paint, Pinta, Artweaver, etc. to 3D Builder and Blocks CAD which can draw 3D objects. Therefore, we put 3D pictures into the paper to increase the reader's understanding of the experiment and device. We take " Determination of Nitrite Ion in environment analysis with a Paper-Based Microfluidic Device" and "Detecting Glucose Levels in Blood Plasma and Artificial Tear by Au(I) Complex on the Carbopol Polymer: A Microfluidic Paper-Based Method". The third part is " Development of a paper-based near-infrared emitting Iridium(III) complex for glucose detection in human blood ". Near-infrared (NIR) dyes have attracted increasing attention for their potential applications in optical imaging in vivo and medical diagnosis. The diagnostic window falling in the NIR range from 650 to 900 nm surpasses the visible region and allows for bioimaging with minimal interference from tissue autofluorescence, reduced light scattering, and high tissue penetration. Ir(III) complexes with mixed ligands in particular have been the subject of considerable interest because of their bright photoluminescence efficiency, long luminescence lifetime, and excellent color tuning. The strong spin–orbit coupling induced by iridium(III) promotes an efficient intersystem crossing from the singlet to the triplet excited state manifold, which then facilitates strong electroluminescence by harnessing both singlet and triplet excitons after the initial charge recombination. Despite intense research using transition metal complexes for glucose detection, application of a glucose biosensor utilizing NIR-emitting Iridium complexes is few. For pursuing high-efficiency NIR-emitting iridium(III) complexes as glucose sensing, we cooperate with Prof. Sergey P. Tunik in Russia. NIR-emitting iridium(III) complex, Ir2, which exhibits NIR emission with a maximum peak at 620 nm. In this study, optical microfluidic paper analytical devices (μ-PADs) for glucose detection from whole blood samples with a small sample volume have been developed on a single paper. In the proposed method, a mushroom-shaped analytical device contains a sample inlet zone and a detection zone. When blood is dripped onto the inlet region of a μPAD, the plasma diffuses to the detection region. The detection region is implanted with a metallic three-dimensional (3D) metal complex hydrogel vehicle. The gel vehicle consists of a complex that responds to oxygen changes and glucose oxidase (GOx) immobilized inside the gel as a bioactivity preservative. The phosphorescence of the complex is enhanced by oxygen consumed by detection of glucose. The influences of alginic acid concentration, calcium chloride concentration, pH and acid buffer concentration are also discussed.