| Summary: | 博士 === 靜宜大學 === 應用化學系 === 107 === Humans have planted a large amount of fruit as a cash crop, but in addition to serving food and various processed products, a large amount of waste is also produced. These wastes include peels, pits, stems, leaves, etc. that are not commonly used for consumption. These wastes are natural products, and many of them have been used for medical purposes or other purposes since ancient times.
Hylocereus polyrhizus cultivation started in Taiwan around the 1980s. The pulp of the fruit is edible and contains small, black, and soft seeds. The peel of the fruits are covered with bracts. The H. polyrhizus fruit is known to be rich in nutrients and minerals. To evaluate the potential applications of the agricultural wastes of H. polyrhizus, the stem, peel, and flower of H. polyrhizus were extracted with solutions of ethanol and water mixed in different ratios. Data was collected for the H. polyrhizus extract including the yield of total phenolics, the total flavonoids, and antioxidant activity, as determined by the 2-2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2`-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay. The protective effects of H. polyrhizus extract on DNA was investigated using an assay with the pUC119 plasmid. The cell proliferation and migration effects were evaluated in the NIH-3T3 fibroblast cell line.
The greatest yield of extract from the stem of H. polyrhizus was 44.70±1.77% (w/w) which was obtained using 50% aqueous ethanol and the greatest yield of extract from the peel was 43.47±1.95% (w/w) using distilled water. The stem extract, which was prepared with 95% aqueous ethanol, had the highest composition of phenolics (8.16±0.45%) and flavonoids (0.87±0.08%) as well as the best DPPH radical scavenging activity (IC50 = 224.00±14.81 µg/mL). The stem extract had excellent ABTS radical scavenging activity (IC50 = 46.05±3.07 µg/mL) as well. The stem, peel, and flower extracts, which were prepared using 95% aqueous ethanol, showed excellent results in protecting themselves from DNA damage, the DNA protect ability were 59.54%, 67.57% and 51.47%, respectively. They are better than the effect of 0.3 mg/mL ferulic acid (44.29%). None of the extracts were able to promote cell proliferation at concentrations of 0.25 mg/mL to 2 mg/mL in a 24 h period. The 1 mg/mL stem and flower extracts in 95% aqueous ethanol promoted considerable cell migration after a 24 h period.
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