| Summary: | 碩士 === 靜宜大學 === 食品營養學系 === 107 === Probiotics can improve intestinal health, prevention of constipation, cancer, cardiovascular disease, hypertension and other fuction. The purpose of this study was to investigate the effects of probiotics on anti-aging and learning memory ability in senescence-accelerated mice(SAMP8). Three months old male and female SAMP8 mices were divided into five groups, control group and 1.03×109cfu/kgBW/day Lactobacillus plantarum, 5.13×109 cfu/kgBW/day Lactobacillus plantarum, 5.13×109 cfu/kgBW/day Lactobacillus paracasei, 5.13×109cfu/kgBW/day Bifidobacterium lactis for 13 weeks. Food intakes, water consumption and body weight were recorded every week. Open field test and grading score were measured in 11th week, forelimb grip strength test was estimated in 12th week, and single-trail passive avoidance test and active shuttle avoidance test in evaluation of learing and memory ability before scarificed. The biochemical parameters of serum were analyzed and organ weight were recorded after scarificed. The antioxidative enzyme activities were estimated in the liver and brain, the 8-hydroxy-2' -deoxyguanosine (8-OHdG) were tested about the DNA damage the β-amyloid of brain was observed. The result showed that food intakes, water consumption, body weight change, open field test and biochemical of serum were no significant difference among the groups. In forelimb grip strength test female mice groups had no significant difference among the groups, but in the male mice experimental groups about Lactobacillus plantarum high dose group and Bifidobacterium lactis had significantly higher than control group(p<0.05). The grading score of experimental groups had significantly loewrer than control group in the total scores (p<0.05). Results about single-trail passive avoidance test and active shuttle avoidance test indicated the experimental groups had significantly better learning memory ability than control group (p<0.05). Probiotics-treated SAMP8 mice had significantly decrease about TBARS contents in the liver and brain (p<0.05).Activities of antioxidant enzyme, including SOD, catalase in the experimental groups were significantly higher than control group (p< 0.05). Besides, oral administration of probiotics could also decrease the 8-OHdG contents and the situation about β-amyloid protein deposition in the brain.
In summary, oral administration of probiotics can enhance the antioxidant enzyme activities, reduce oxidative stress damage, improve learing and memory ability and retard the aging process.
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