| Summary: | 碩士 === 靜宜大學 === 化粧品科學系 === 107 === Hydrosol, also known as hydrolat, refers to an aqueous distilled solution obtained by extracting plant essential oil by a large amount of steam distillation. Water is a high proportion of solvents in most cosmetics. Therefore, the we expect that hydrosol can replace the water in cosmetic, and has both aroma and other effective efficacy. The purpose of this study was to evaluate the safety and efficacy of eight hydrosols Aloysia triphylla, Cymbopogon citratus, Cinnamomum camphora, Chamaecyparis formosensis, Cinnamomum osmophloeum, Melaleuca alternifolia, Melissa officinalis and Mentha piperita swiss in cytotoxicity, melanin content, anti-inflammatory, anti-oxidation and the stability of the products containing hydrosol to explore the application of hydrosol in cosmetics.
The efficacy test of each cell was carried out by selecting the concentration of non-cytotoxic hydrosol. The results showed that 1.0% A. triphylla hydrosol and 1.0 % C. citratus hydrosol had significant increased melanin content of B16F10 cells. 0.4 % C. camphora hydrosol, C. formosensis hydrosol, M. alternifolia hydrosol and M. officinalis hydrosol had significant reduced the nitric oxide of Raw 264.7 cells induced by LPS. 0.6 % C. osmophloeum hydrosol had significantly increased the melanin content of B16F10 cells and significantly reduced the nitric oxide of Raw 264.7 cells induced by LPS. 0.05~0.2 % M. piperita hydrosol had significant reduced the nitric oxide of Raw 264.7 cells induced by LPS. Content product containting hydrosol can stability by centrifugation test as well as be maintained for more than 3 months by constant temperature test, and can be maintained in the cycle temperature test at least 7 cycles. All stability tests do not cause discoloration, odor or other deterioration, and the aerobic plate count will also meet the hygiene standards.
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