Evaluating efficacy of the Vernonia amygdalina by the extraction conditions of Taguchi experiment design

• Main Authors: WU,LING-LING, 吳鈴鈴
• Other Authors: HUANG,SHU- LING
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/4gwc79

碩士 === 國立聯合大學 === 化學工程學系碩士班 === 107 === In this study, evaluating efficacy of the Vernonia amygdalina by the extraction conditions of Taguchi experiment Design for hot reflow extraction and ultrasonic extraction methods. Besides, the total phenolic content and total flavonoid content were used as experimental indicators by the samples with the highest total polyphenols/flavonoids contents. The evaluating efficacies include antioxidant, moisturizing, anti-bacterial, anti-inflammatory and anti-cancer. The results showed that the hot reflux extraction of VA sample has the highest total phenolic content of 20.6 ± 0.15 mg GAE / g. Using DSC to measure the VA sample by hot reflux method, the freezing bound water content of the sample was 16.37%, which was better than the control 5% glycerol (8.97%) and 10% glycerol (10.60%). In addition, both the VA samples by hot reflux and ultrasonic extractions present a good antibacterial; besides, the ultrasonic VA has a best antibacterial effect for Escherichia coil than that of streptomycin sulfate. At low concentrations, VA extract has good anti-inflammatory effects on mouse macrophages (Raw264.7).More worth mentioning, the samples by the hot reflux has an excellent anti-cancer effect for the colorectal cancer cell (HT-29) than that of 5-FU chemotherapy drug.