Preparation of Thin Films Containing Amine and Carboxylic Functionalities by Plasma Polymerization for Applications in Biomaterials

• Main Authors: Tai-Chun Tsai, 蔡岱均
• Other Authors: Meng-Jiy Wang
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/u4893j

碩士 === 國立臺灣科技大學 === 化學工程系 === 107 === The applications of biomaterials have drawn increasingly attention in fields of regenerative medicine and tissue engineering although some complications might occur. For example, the deposition of proteins or different biomolecules on the surface of the biomaterials which are in contact with personnel might lead to discomfort of patients. In order to overcome the aforementioned problems, the coatings of thin films on the surface of the biomaterials became an important issue for the applications in the fields of biomedicine. The research goal of this thesis is to prepare plasma polymerized thin films containing amine and carboxylic functional groups by introducing allylamine (AAm), pyrrole (Py) and acrylic acid (AAc) as precursors. By adjusting different applied power, plasma deposition time, and the orders of introducing precursors, the thickness of the plasma deposited thin films, surface wettability and functional groups, biocompatibility, and the antifouling properties of the resultant thin films will be discussed. At the first part of the thesis, in order to promote the surface wettability and to decrease the biofouling problems of contact lens, pp(AAc+AAm) thin film was prepared. The surface wettability of pp(AAc+AAm) thin film was 10˚, comparing with the water contact angle (WCA) of 46˚ on untreated glass. The deposition of pp(AAc+AAm) for 30 mins for glass allowed to reduce 52.3 % and 26.9 % adsorption for lysozyme (LYS) and bovine serum albumin (BSA), comparing with pristine contact lens, respectively. The cell density of L-929 mouse fibroblast on pp(AAc+AAm) thin films after 24 h incubation time showed the similar result when compared with the pristine contact lens, which indicated that pp(AAc+AAm) thin film shows good biocompatibility. At the second part of the thesis, in order to optimize the parameters for the deposition of conductive thin films, pyrrole was chosen as the precursor. Plasma-polymerized pyrrole (pp(Py)) thin films were deposited on glass with the applied power at 5 W, 10 W, and 20 W. The surface wettability was carried out by measuring the water contact angle which increased from 46˚ to 60˚ after the deposition of pp(Py) on glass. The WCA stayed unaltered water with longer deposition time. LDH assay results revealed that the cell density for L-929 mouse fibroblast on pp(Py) thin films decorated glass increased 155 %, 106 % and 170 %, when compared with the pristine glass at the applied power for 5 W, 10 W and 20 W, respectively. In addition, from optical microscope image revealed that the cell morphology was in fibroblast shape. When the applied power and the film thickness of pp(Py) thin films were controlled at 20 W and 10 nm, the pp(Py) thin film showed the highest current density (982 A/cm2) by iodine doping for 6 h. ESCA analyses showed that the N1s signal appeared after pp(Py) thin film was deposited on Si wafer. Moreover, I3d signal was found after iodine doping, which confirmed that pp(Py) thin film was successfully deposited on Si wafer by plasma polymerization and can be iodine doped. For the third part of the thesis, in order to prepare thin films containing both amine and carboxylic functional groups, pyrrole and acrylic acid were chosen as the precursors. The surface wettability can be modulated by depositing different thin films as the top layer. When pp(AAc) was deposited as the top layer, the WCA decreased from 46˚ (pristine glass) to 10˚. Alternatively, when pp(Py) was deposited as the top layer, the WCA increased to 53˚. By depositing pp(AAc+Py) on glass, the WCA was 30˚, showing the potential hybrid properties of pp(AAc+Py), comparing with pp(Py) and pp(AAc). The antifouling property was carried out by using LYS and BSA as protein models. Compared with pristine glass, the LYS adsorption on pp(Py/AAc)/glass decreased 44 %, comparing with the prisitnie glass. The BSA adsorption on pp(AAc/Py)/glass, pp(Py/AAc)/glass and pp(AAc+Py)/glass decreased to 71 %, 41 % and 40 %, respectively, which showed that plasma polymerized amine and carboxylic functional groups on glass demonstrated the antifouling property. Moreover, the surface charge of pristine glass was -43.2 mV and increased to -41.9 mV after the depositing pp(AAc/Py) on glass. For pp(Py/AAc)/glass and pp(AAc+Py)/glass, the surface charge were -28.9 mV and -11.2 mV. The biocompatibility tests for pp(AAc/Py)/glass, pp(Py/AAc)/glass, and pp(AAc+Py)/glass showed that the L-929 mouse fibroblast cell density increased 40 %, 50 % and 107 % comparing with pristine glass, which concluded plasma polymerized amine and carboxylic functional groups possess the potential for the biomaterial applications.