Poly(N-isopropylacrylamide-co-acrylamide) core – silica shell thermal-sensitive hybrid nanoparticles for drug delivery

• Main Authors: Jian-Wei Huang, 黃建維
• Other Authors: Chorng-Shyan Chern
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/93tmvr

碩士 === 國立臺灣科技大學 === 化學工程系 === 107 === In this research, the thermal-sensitive material NIPAM was used, and Acrylamide (AM) was used as a free radical addition polymerization. The content of AM was adjusted to prepare different ratios of poly(N-isopropylacrylamide-co-acrylamide) (P(NIPAM-co-AM)), and as a backbone. Adding 3-glycidyloxypropyltrimethoxysilane (GLYMO) and Tetraethyl orthosilicate (TEOS) Tetraethyl orthosilicate (TEOS), use GLYMO and AM for ring-opening reaction, and use GLYMO and TEOS by Sol-Gel Reaction to get the final product The thermal-sensitive hybrid nanoparticles P(NIPAM-co-AM)_Silica. It was used and the feasibility of applying the nanoparticles to the drug delivery system was evaluated. Because the hybrid nanoparticle synthesized in the research is temperature sensitive. When the temperature is lower than the low critical solution temperature (LCST), the polymer chain is swelling, and the nanoparticles are hydrophilic. At ambient pH 7.4, the material will have a negative charge and can interact with the positively charged DOX via electrostatic interaction. Conversely, if the temperature is higher than LCST, it will be hydrophobic. We use the property to do load drug. It is carried out at low temperature, the drug coating efficiency is about 45 - 55% for different AM ratios. The drug release is carried out under different pH value, such as pH 7.4, pH 6 and pH 5, and the environment is simulated at 37 °C. The hybrid nanoparticles will shrink and the drug is coated in the core. However, because the drug will be squeezed internally, and the acidic environment will provide protons, the drug will be released by mass transfer, and the release rate in 120 hours, different AM ratios, at pH 7.4, pH 6 and pH 5 reached 40%, 70% and 80% respectively. Finally, the drug-coated composite nanoparticles were co-cultured with cancer cells (Hela cells), and the cancer cells were analyzed for cytotoxicity and phagocytosis. In summary, exploring the application value of this hybrid material in drug delivery system for cancer treatment.