Protein Extraction from Their Aqueous Solutions by Using New Ionic Liquids

• Main Authors: Yi-Chen Wu, 吳怡臻
• Other Authors: Ming-Jer Lee
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/xd4jwh

碩士 === 國立臺灣科技大學 === 化學工程系 === 107 === In the present study, we synthesize eight new Good’s buffer ionic liquids(GB-ILs) from Good’s buffers (Bicine, MOPS, TAPS or Tricine) as anion part with tetrabutyl-ammonium (TBA) or tetrabutyl-phosphonium (TBP) as cation part. Sodium citrate was used as a salting-out agent to form the ionic liquid aqueous biphasic systems (IL-ABS) for protein extraction. Firstly the liquid-liquid phase boundary of each IL-ABS was determined experimentally by cloud-point method. We obtain the mass compositions of the systems at the volume ratios of the ionic liquid-rich phase to the salt-rich phase around 1:1, 1:4, and 1:8. Using bovine serum albumin and lysozyme as the target materials, explore the extraction efficiencies at 298.2 K and pH = 7 when the volume ratios of the ionic liquid-rich phase to the salt-rich phase is 1:1, 1:4, and 1:8. In this study, we also used various biophysical techniques, including UV-visible, fluorescence, Fourier transforms infrared spectroscopy (FTIR) and dynamic light scattering (DLS) for analyzing the stability of the extracted proteins.