Chemical Optimization for Functional Ligament Tissue Engineering

• Main Authors: Chun-Wei Chang, 張峻瑋
• Other Authors: Pen-Hsiu Chao
• Format: Others
• Language: en_US
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/baw36b

碩士 === 國立臺灣大學 === 醫學工程學研究所 === 107 === The limited repair ability of anterior cruciate ligament (ACL) prompted research in ligament tissue engineering to be a promising treatment strategy. We aim to optimize current issues of poor infiltration, poor ECM deposition and limited construct size. Collagen coating improved cell infiltration and resulted in homogeneous ligament cellularity on the densely fibrous PLLA scaffold. We found that the sequential growth factor supplementation of fibroblast growth factor-2 (FGF-2) and transforming growth factor beta-1 (TGF-β1) increased ACL cellularity and collagen deposition. To increase construct size, we laminated two layers of scaffolds with fibrin, which further improved collagen deposition and mechanical properties. Future study will focus on the interaction between fibrin gel and growth factors.