| Summary: | 碩士 === 國立臺灣大學 === 園藝暨景觀學系 === 107 === Arbuscular mycorrhizal (AM) fungi which help plants to absorb mineral nutrients from rhizosphere and enhance their tolerance to abiotic and biotic stresses are applied as the biofertilizer for crop production . Medicago truncatula MtNF-YC6 and C11, two highly AM symbiosis-induced genes, are conserved in the genome of AM host plant species. MtNF-YC6 and C11 which belong to C subfamily of Nuclear Factor Y (NF-Y) transcription factor family usually form a trimer with A and B subunits to regulate downstream gene expression. This study aims to investigate the roles of MtNF-YC6 and C11 in AM symbiosis. Through yeast two-hybrid assay, MtNF-YA3, A4, A8, B12 and B17 were identified as potential interacting partners of MtNF-YC6 and C11. The subcellular localization of these proteins were examined in the leaves of Nicotiana benthamiana by using agroinfiltration, and the results suggested that MtNF-YB12, B17, C6 and C11 were localized in the cytosol and the nucleus. Intriguingly, the coexpressing either B12 or B17 with C6 or C11 changed the localization of most proteins to the nucleus. Four MtNF-YC6 and C11 double knockdown Medicago plants were generated by RNA interference techniques. However, only one line showed lower fungal colonization rate, arbuscule formation and the reduction of AM symbiosis marker gene (MtPT4 and MtBCP) expression compared to empty vector controls. In conclusion, identification of the interacting partners of MtNF-YC6 and C11 and their subcellular localization through this study provide clues for studying the roles of MtNF-Y complex in AM symbiosis in the future.
|
|---|
