| Summary: | 碩士 === 國立臺灣大學 === 園藝暨景觀學系 === 107 === Artemisia argyi Levl. Et Vant. with strong aroma is a common Chinese traditional medicine. However, there are plenty similar species and the index of taxonomy is not clear. We compared 5 species of wormwood in Taiwan with the leaf morphology and volatile compounds by GC/MS analysis. With the results of volatiles compounds, we established the preliminary fingerprints for these 5 vareities. We also discussed the total phenolic contents and DPPH radical scavenging capacity of wormwood. The impact of temperature on volatile and its functional efficacy was to be evaluated.
We investigated the leaf characters of 5 Artemisia argyi varieties and used solid phase microextraction (SPME) with gas chromatography-mass spectrometry (GC-MS) to analyze the fresh leaf volatile constituents. The lobes of leaf, the leaf margin and the stipule were the major characteristics to distinguish 5 wormwood varieties. There were 27 volatile compounds identified. The main aroma components were 1,8-cineole, α-pinene, thujoner, caryophyllene and myrcene. Using the radar diagram for the distribution in kinds of volatile organic compound, the volatiles in wide-leaf wormwood showed the most different from others.
The effect of the growth temperature on wild wormwood were investigated. The 4 temperate sets were day/night 30/25℃, 25/20℃, 20/15℃and 15/13℃, respectively. The wild wormwood showed the highest natural height in 30/25℃, and there were most leaf numbers of the wild wormwood in 25/20℃environment. The most relative contents of aroma components in fresh leaves were α-pinene (54.69%) and myrcene (7.18%) in 30/25℃environmen. In 15/13℃, the wild wormwood had more β-ocimene and β-pinene, and their relative contents were 7.38% and 21.54% respectively. The wild wormwood growing in 30/25℃ had 33.49 mg/g phenolic compounds content at 30/25℃environment , and it was the highest content in all treatments. The DPPH radical scavenging activities in all reattments were 75.47%-77.96%, and ther was no significant difference.
Integrating the data of the leaf characters and chemical fingerprints of Artemisia argyi, it can be an useful classification tool. Growth temperatures had significant effects on the growth and phenol content of wild wormwood, but not on the DPPH radical scavenging capacity.
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