| Summary: | 碩士 === 國立臺灣大學 === 食品科技研究所 === 107 === The pure compounds isolated from the fruit or other parts of Chinese olive (Canarium album L.) have been proven to have many physiological functions such as liver protection, and anti-oxidation. However, little is known about the effects of Chinese olive extracts on cancer cells. Previous research in our lab indicates that the ethyl acetate fraction of ethanol extract of Chinese olive water extract residue (COE) exhibits anti-tumor effect in both cell and animal models. In the early stage of tumor formation, cancer cells rapidly proliferate, thus makes the inner cancer cells lack the supply of oxygen and glucose. Therefore, this study intends to investigate the anti-tumor effect of COE in either high or low glucose. We have treated HCT116 cells with different concentration of COE at different glucose concentrations, and examined the efficacy of COE on cell viability, colony formation, and migration. We found that COE at effectively inhibit HCT116 cell growth in high glucose. However, in low glucose, the inhibitory effect is rather poor. Also, in clonogenic assay, less inhibitory effect of COE in low glucose was observed. Furthermore, in low glucose, COE could further activate AMPK and the consequent inactivation of ACC as well as the enhancement of fatty acid oxidation (FAO) related genes. This phenomenon was less significant in high glucose, which caused opposite growth effects in different glucose levels upon COE treatment. COE dose exceeds 400 μg/ml would damage mitochondrial and leads to apoptosis, which can no longer rescue cells by the additional function of COE on increasing FAO mediated energy production. Although more and more studies point out that phytochemicals have the potential to inhibit cancer cells, here the results suggested that the opposite effects upon different concentration of glucose should be considered before using phytochemicals for cancer therapy.
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