Preparation and functions of resveratrol-ferulic acid loaded nanostructured lipid carrier system

• Main Authors: Low Yan Ling, 劉彥伶
• Other Authors: Been-Huang Chiang
• Format: Others
• Language: en_US
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/k3xt63

碩士 === 國立臺灣大學 === 食品科技研究所 === 107 === Surging consumer demand for skin care product that are free from harmful chemicals has encouraged the development of product derived from natural sources. Among all the product benefits skin lightening has gained a lot of attention in the last years, especially in Asian communities. Topically applied product, however, often suffer from low penetration of active compounds into skin due to excellent barrier of stratum corneum. In an attempt to overcome these problems, this study has developed a nanostructured lipid carriers (NLC) system encapsulating both hydrophilic ferulic acid and lipophilic resveratrol. Both of these two compounds are known to be able to inhibit melanogensis in skin. Natural derived ingredients including illipe nut butter, capric triglyceride, polyglyceryl 4 isostearate, and polyglyceryl 3 methylglucose distearate were chosen to fabricate NLC. A full factorial design was used to optimize the concentration of polyglyceryl 3 methylglucose distearate and duration of ultrasonication during second stage of emulsification. Results showed that 2.56% of polyglyceryl 3 methylglucose distearate and 6 minutes sonication time were suitable to produce stable NLC system with mean diameter of 375.37 ± 90.26 nm, 94.29% ± 1.39 stability, 85.49% ± 8.45 ferulic acid encapsulation efficiency, and 85.57% ± 1.39 resveratrol encapsulation efficiency. The ex vivo penetration study revealed that NLC could increase the penetration of resveratrol for about 29.31% in porcine back skin. The in vivo tape stripping penetration study indicated that the NLC increased the cumulative penetration of ferulic acid and resveratrol by 17.23% and 27.19% respectively as compared to the free compounds. The in vitro antimelanogenic effects of ferulic acid and resveratrol was demonstrated by treating B16F10 mouse melanoma cells with different amount of these phytochemicals loaded NLC before and after exposure to UVA radiations. These findings suggested that NLC made by natural derived ingredients is a promising topical delivery system that had a potent enhancement effect for ferulic acid-resveratrol penetration into skin.