| Summary: | 碩士 === 國立臺灣大學 === 光電工程學研究所 === 107 === Actinic keratosis (AK) is a common cutaneous neoplasm consisting of proliferation of atypical epidermal keratinocytes resulting from severe solar damage. AKs are important because of their potential to develop into invasive cutaneous squamous cell carcinoma (SCC) with the highest incidence in the aged population. AK patients usually have multiple AKs. To study the diagnosis of AK, in vivo harmonic generation microscopy (HGM) was proposed as the imaging tool. HGM provides submicron resolution crucial for histopathological examination and causes little optical damage in bio-tissues. With HGM, this thesis is aimed at seeking out the consistent cell morphology in comparison with the H&E stained histopathological images. Five Asian volunteers (2 females and 3 males) aged from 70 to 93 years old with AK were enrolled in this study. It contained 1 ex vivo and 4 in vivo examinations. Due to the clinical demands, we also developed movable HGM system with light guide in this study. HGM and H&E images of AK both show parakeratosis and hyperkeratosis in the stratum corneum (SC), irregular acanthosis, abnormal architecture and pleomorphism of cells and nuclei in the stratum spinosum (SS), crowding of keratinocytes, abnormal architecture and pleomorphism of cells and nuclei in the stratum basale (SB), and solar elastosis in the dermis. Moreover, we also found dendritic-like cells in AK through HGM images. Our case study indicates that noninvasive HGM may become an alternative to invasive biopsy in the diagnosis of AKs and also helps physician to improve follow-up treatment assessment.
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