| Summary: | 碩士 === 國立臺灣大學 === 分子與細胞生物學研究所 === 107 === The flowering time of Arabidopsis thaliana is regulated by the crosstalk of environmental and endogenous factors. FLOWERING LOCUS C (FLC), is the main repressor of flowering in Arabidopsis. Once the plant meets suitable conditions to propagate, FLC would be repressed and the flowering genes are activated. The vernalization and autonomous control, are two negative pathways inhibit the expression of FLC. Under the vernalization, the prolonged duration of low temperature silences FLC expression via Polycomb Repression Complexes (PRCs)-mediated irreversible histone methylation. In autonomous pathway, the FLC is silenced by histone methylation mediated by non-coding RNAs. The above repressive mechanisms action on the various regions of FLC locus, currently, if the FLC could be repressed via the promoter region remains unknown. It has been shown that the bzip16 mutant is late-flowered due to the enhancement of FLC expression. Also, bZIP16 associates with the FLC promoter in vivo, therefore, bZIP16 could be a repressor acting on the FLC promoter. To study the regulatory role of bZIP16 in flowering time control, this study generates an inducible system for bZIP16 overexpression and the reporter construct for FLC promoter. Furthermore, a bZIP16-binding motif, G-box (CACGTG), is found in the region that bZIP16 associated with the FLC promoter, we also generated a reporter construct to investigate the role of G-box in the promoter. All the effector and reporter constructs are established in binary vectors that will be further applied to transient assays and generate transgenic plants.
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