The Resonance Properties and Biosensing Application of Metal- Dielectric-Metal Coated Periodic Nanowire
碩士 === 國立臺灣海洋大學 === 光電科學研究所 === 107 === This paper describes the use of metal-dielectric-metal processes to make bio-sensing chips. Base on the theory of surface plasmon resonance. Making a metal- dielectric-metal film structure on a periodic injection molding substrate, an accurate design of transm...
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ndltd-TW-107NTOU56140022019-05-16T01:44:46Z http://ndltd.ncl.edu.tw/handle/uv3thd The Resonance Properties and Biosensing Application of Metal- Dielectric-Metal Coated Periodic Nanowire 週期性奈米線披覆金屬-介質-金屬結構之共振特性探討及生醫感測應用 Wang, Yu-Kai 王昱凱 碩士 國立臺灣海洋大學 光電科學研究所 107 This paper describes the use of metal-dielectric-metal processes to make bio-sensing chips. Base on the theory of surface plasmon resonance. Making a metal- dielectric-metal film structure on a periodic injection molding substrate, an accurate design of transmission and reflection spectra, reproducibility, high throughput, high sensitivity, and instant detection. In this experiment, different thicknesses of metal layer are coated using thermal evaporation, and metal thickness is measured using a film thickness profiler (α-step) to adjust the metal and dielectric film thickness parameters. Followed by a transmissive optical system, the periodic injection molding is measured to detect changes in wavelength and intensity of the microchip. The wavelength of the metal- dielectric -metal thin film structure is designed to be coupled with the surface plasmon resonance wavelength. Using the microfluidic channel system to carry out refractive index test with different environmental concentrations of glycerol water, it is found that when the incident light source illuminates the sample, there will be a certain angle to increase the wavelength sensitivity and the wavelength shift to the blue shift (short wavelength). In order to confirm and explain the blue shift phenomenon, where negative refraction is used to explain the problem and to make samples of repetition for analysis. In the part of the biological experiment, the surface was modified with cysteamine and glutaraldehyde solution, and the rate of coating was improved without removing the conditions in the modification step. Subsequently, the microfluidic channel system was sealed on the chip, and bovine serum albumin (BSA) and immunoglobulin (IgG) biological solution was used. Both were separately formulated with different concentrations for analytical measurement. On the metal-dielectric-metal detection chip, the biochip for detection application can be enhanced, and its very research and development for the detection biosensing chip which result the blue shift phenomenon of the wavelength band in the future. Keywords: Surface Plasmon Resonance (SPR), Nanostructure, Multilayer Structure, Non-labeled, High Throughput, Biosensing, Injection Molding Microchip. Wei, Pei-Kuen Chiang, Hai-Pang 魏培坤 江海邦 2019 學位論文 ; thesis 62 zh-TW |
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碩士 === 國立臺灣海洋大學 === 光電科學研究所 === 107 === This paper describes the use of metal-dielectric-metal processes to make bio-sensing chips. Base on the theory of surface plasmon resonance. Making a metal- dielectric-metal film structure on a periodic injection molding substrate, an accurate design of transmission and reflection spectra, reproducibility, high throughput, high sensitivity, and instant detection.
In this experiment, different thicknesses of metal layer are coated using thermal evaporation, and metal thickness is measured using a film thickness profiler (α-step) to adjust the metal and dielectric film thickness parameters. Followed by a transmissive optical system, the periodic injection molding is measured to detect changes in wavelength and intensity of the microchip.
The wavelength of the metal- dielectric -metal thin film structure is designed to be coupled with the surface plasmon resonance wavelength. Using the microfluidic channel system to carry out refractive index test with different environmental concentrations of glycerol water, it is found that when the incident light source illuminates the sample, there will be a certain angle to increase the wavelength sensitivity and the wavelength shift to the blue shift (short wavelength). In order to confirm and explain the blue shift phenomenon, where negative refraction is used to explain the problem and to make samples of repetition for analysis.
In the part of the biological experiment, the surface was modified with cysteamine and glutaraldehyde solution, and the rate of coating was improved without removing the conditions in the modification step. Subsequently, the microfluidic channel system was sealed on the chip, and bovine serum albumin (BSA) and immunoglobulin (IgG) biological solution was used. Both were separately formulated with different concentrations for analytical measurement. On the metal-dielectric-metal detection chip, the biochip for detection application can be enhanced, and its very research and development for the detection biosensing chip which result the blue shift phenomenon of the wavelength band in the future.
Keywords: Surface Plasmon Resonance (SPR), Nanostructure, Multilayer Structure, Non-labeled, High Throughput, Biosensing, Injection Molding Microchip.
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author2 |
Wei, Pei-Kuen |
author_facet |
Wei, Pei-Kuen Wang, Yu-Kai 王昱凱 |
author |
Wang, Yu-Kai 王昱凱 |
spellingShingle |
Wang, Yu-Kai 王昱凱 The Resonance Properties and Biosensing Application of Metal- Dielectric-Metal Coated Periodic Nanowire |
author_sort |
Wang, Yu-Kai |
title |
The Resonance Properties and Biosensing Application of Metal- Dielectric-Metal Coated Periodic Nanowire |
title_short |
The Resonance Properties and Biosensing Application of Metal- Dielectric-Metal Coated Periodic Nanowire |
title_full |
The Resonance Properties and Biosensing Application of Metal- Dielectric-Metal Coated Periodic Nanowire |
title_fullStr |
The Resonance Properties and Biosensing Application of Metal- Dielectric-Metal Coated Periodic Nanowire |
title_full_unstemmed |
The Resonance Properties and Biosensing Application of Metal- Dielectric-Metal Coated Periodic Nanowire |
title_sort |
resonance properties and biosensing application of metal- dielectric-metal coated periodic nanowire |
publishDate |
2019 |
url |
http://ndltd.ncl.edu.tw/handle/uv3thd |
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