Reproductive biology and complete mitochondrial genome of endemic amphidromous goby Rhinogobius formosanus (Teleostei: Gobiidae) from the Masu River, Northern Taiwan

• Main Authors: Tonisman Harefa, 方威廷
• Other Authors: I-Shiung Chen
• Format: Others
• Language: en_US
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/6256xv

碩士 === 國立臺灣海洋大學 === 海洋生物研究所 === 107 === The freshwater goby Rhinogobius formosanus Oshima, 1919 is one of endemic fish, listed as being near threatened species and has a narrow distribution in the northern Taiwan although it belongs to amphidromous species. Present study aimed to investigate reproductive aspects through monthly collection from March 2018 to March 2019 from the Masu River, New Taipei City, North Taiwan. Overall 355 fish gobies were examined including 180 females and 155 males. Total length range for females was 21.17 to 70.76 mm and for males was 28.54 to 72.12 mm. The overall sex ratio between female and male was 1.16: 1. Spawning season was estimated to occur in February-April, based on the monthly change of GSI. Absolute fecundity of mature female ranged from 806 to 2955 with an average 1,489 ± 468, showing a significant increase with length and body weight of fish. Oocyte size averaged was 0.51mm. Female length at first maturity was 44.18mm and for male was 47.48mm. Length-weight relationship described growth pattern of this fish goby was allometric negative. Fish mitochondrial genome is very important to understand their molecular evolution and phylogenetic relationships. The complete mitochondrial genome sequence of R. formosanus was 16,502 bp in length, and contained 37 genes with 22 tRNAs, 2 rRNAs, 13 PCGs, one origin of replication (OL) and one control region in this mitogenomes. The overall of base composition was 26,2 % for A, 30,5% for C, 25,6% for T and 17,7% for G. Phylogenetic tree was constructed with Maximum likelihood and Bayesian Inference method based on complete mitogenome sequences. Both tree topologies highly supported the relationship between R. formosanus and R. brunneus.