| Summary: | 碩士 === 國立臺灣海洋大學 === 水產養殖學系 === 107 === Algae are rich in protein and other bioactive components. The breakage of macroalgae cells which are a noteworthy prevention for cell interruption and hindering essential extraction of the biomolecules are required for complete arrival of bioactive mixes. Therefore, this study has been aimed to evaluate the extraction efficiency of bioactive constituents such as essential pigments and polysaccharides of three types of algae (Acrochaetium sp., Sarcodia suiae and Nostoc commune) as substrates by fermentation.
In stage one experiment, three type of algae (Acrochaetium sp., S. suiae and N. commune) were used as substrates to ferment with Pseudoalteromonas haloplanktis ATCC14393. After crude fragmentation (except Acrochaetium sp.), they were started for fermentation. The concentration of phycobiliproteins, chlorophyll a, reducing sugar and total count of bacteria were measured. Thereafter, the analysis of reducing sugar on three fermented algae were assayed by HPLC analysis. Acrochaetium sp. was carried for further analysis in stage two experiment.
Acrochaetium sp., S. suiae and N. commune fermented by using P. haloplanktis ATCC14393 has produced more bioactive constituents such as phycoerythrin, phycocyanin, allophycocyanin and reducing sugar from the beginning until the end of fermentation. Acrochaetium sp. has produced the highest phycoerythrin with more than 0.1 mg·mL-1 compare to S. suiae and N. commune. After a strong fragmentation (stage two experiment), the content of phycoerythrin has increased to 0.206 mg in each gram of Acrochaetium sp. After strong fragmentation, the residue is taken out to the first phase fermentation which produced 0.296 mg of phycoerythrin after six hours of fermentation. It has produced 0.649 g phycoerythrin in each gram of algae around 48 h in the second phase fermentation. This result shows that with the addition of P. haloplanktis, ATCC 14393 in the fermentation process can extract more phycoerythrin as similar to phycocyanin and allophycocyanin. All of the algae after fermentation have produced mannose under undissociated enzyme condition. Acrochaetium sp., N. commune and S. suiae has produced 70.815 g·mL-1, 56.228 g·mL-1, and 50.347 g·mL-1 of mannose respectively. After enzyme dissociation, Acrochaetium sp. and S. suiae have produced mannose and xylose, while N. commune has given out mannose and others unknown sugar.
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