Sequential Biotransformation of Antcin K by Bacillus subtilis ATCC 6633

• Main Authors: SIAO, YI-YUN, 蕭亦芸
• Other Authors: CHANG, TE-SHENG
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/65ebp9

碩士 === 國立臺南大學 === 生物科技學系碩士班 === 107 === Antrodia camphorata is a parasitic fungal species that is unique to Taiwan. Antcin K is an ergostane type triterpenoid derived from the fruit body of Antrodia camphorata. Three bacteria were known to catalyze the glycosylation of triterpenoids from previous studies. We selected one of the strains Bacillus subtilis ATCC 6633 to biocatalyze Antcin K and analyze its products by ultra-high pressure liquid chromatography. The produced metabolites were purified by preparative high-pressure liquid chromatography to separate four new metabolites. The structure of the compound 25S-antcin K 26-O- -glucoside was identified by mass spectrometry and nuclear magnetic resonance spectroscopy. 25R-antcin K 26-O--glucoside、25S-antcin K 26-O--(6’-O-succinyl)-glucoside and 25R-antcin K 26-O--(6’-O-succinyl)-glucoside. Using 25S-antcin K 26-O--glucoside and 25R-antcin K 26-O--glucoside as precursors for biotransformation, it was proved that 25S-antcin K 26-O--(6’-O-succinyl)-glucoside and 25R-antcin K 26-O--(6’-O-succinyl)-glucoside are composed of 25S-antcin K 26-O--glucoside and 25R-antcin K 26-O--glucoside bio-transformed succinic acid derived from grape glucoside. To the best of this knowledge, this study is the first study on the glycosylation of triterpenoids from Antrodia camphorata, and for the first time, the microbial studies on the biosynthesis of succinic acidification of triterpenoid glycosides.