Evaluation the use of neritid egg capsule and embryonic development to seawater acidification biomarker

• Main Authors: Chen-Hao Chang, 張晨浩
• Other Authors: Li-Lian Liu
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/jpru85

碩士 === 國立中山大學 === 海洋科學系研究所 === 107 === Evaluation the use of neritid egg capsules and embryonic development to seawater acidification biomarker Chen-Hao Chang Advisor：Dr. Li-Lian Liu Department of Oceanography, National Sun Yat-sen University, Kaohsiung, Taiwan, R.O.C. The effects of seawater acidification on marine organisms’ calcification are an important issue recently. In this study, the intertidal Nerita snails are selected to evaluate the potential of using egg capsules and embryonic development as a seawater acidification biomarker. The experiment is divided into three parts. Firstly, the comparison of different pH values (pH 6.0 and 8.0) on the surface erosion of Nerita albicilla egg capsules. Secondly, the comparison of the erosion degree of egg capsules and the embryonic development on N. albicilla and Nerita undata among different locations. Thirdly, the comparison of the erosion degree of egg capsules and the embryonic development on N. albicilla and N. undata by transplant experiments. The CaCO3 structure on the surface of N. albicilla egg capsules in acidic seawater was eroded. Based on the degree of capsule erosion, it was divided into three levels. The erosion degree of capsules was higher in Mailiao than those from Qigu and Taixi. When comparing the embryonic development and erosion at the same time, the erosion degree of capsules from Taixi and Mailiao was relatively higher than the ones from Qigu. The transplant experiments were unsuccessful due to no living embryos was found. In summary, the degree of capsule erosion increased with time in acidic seawater and a lower erosion degree of capsules from Qigu than those from Taixi and Mailiao when embryonic development was also considered. In the future, if larval activity can be examined at the same time, Nerita snails still have the potential to be a biomarker for the study of seawater acidification.