| Summary: | 碩士 === 國立中山大學 === 海洋科學系研究所 === 107 === Combined effects of dietary hydrogenated soybean oil and DHA on the growth and DHA deposition of the grouper (Epinephelus coioides)
Wei-Ching Chang
Advisor: Dr. Houng-Yung Chen
Department of Oceanography, National Sun Yat-sen University,
Kaohsiung 80424 Taiwan
Abstract
This study investigated the combined effects of dietary hydrogenated soybean oil (HSO) and DHA on the growth performance and DHA deposition of the grouper (Epinephelus coioides), An 8-week grow-out experiment, followed by a 4-wk washing-out experiment, was carried out. The grow-out experiment was of a 3×2 factorial design, in which soybean oil was replaced by HSO at 0, 50, or 100%, and DHA was supplemented at 3.8 (low) or 10.8 (high) g/kg which is lower or higher than the requirement level, respectively. Juvenile groupers with an initial weight of 64.3 g were stocked in flow-through tanks at 20 fish/tank. Each dietary treatment was randomly assigned to four replicate tanks (N=4). The fish were hand fed twice daily to apparent satiation. The results of the grow-out experiment showed that fish growth was significantly higher in the high DHA group than the low DHA group, and there was no difference among the HSO groups. Body DHA deposition was increased with increasing dietary HSO level. In the washing-out experiment, fish growth was increased with increasing dietary HSO level in the grow-out experiment. DHA deposition was increased with the increasing HSO level in the grow-out experiment. The present results show that dietary inclusion of HSO was associated with body DHA deposition and growth in the grower regardless of dietary DHA levels. While DHA deposition was increased with increasing dietary HSO levels for both experiments, HSO level did not affect the growth of the grouper in the grow-out experiment, but promoted fish growth in the washing-out experiment.
Key words: hydrogenated soybean oil, DHA, washing-out, growth performance, lcPUFA
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