| Summary: | 碩士 === 國立高雄師範大學 === 化學系 === 107 === Surfactant-assisted dispersive liquid-liquid microextraction combined with on-line concentration - polymer stacking ( SA-DLLME-Polymer stacking ) in capillary electrophoresis was determined to be a simple and sensitive analytical method for detecting R,S-Mirtazapine and its metabolite R,S-Desmethyl mirtazapine, R,S-8-Hydroxy mirtazapine in human urine and serum. The electrophoretic analyses were carried out in 10 mM Phosphate buffer solution at pH 3.0 containing 0.9 % [Poly (diallyldimethylammonium chloride)] ( PDDAC ) and 20 mM Dimethyl-β-cyclodextrin.Because PDDAC exhibits viscosity, the effect of the polymer stacking and detection sensitivity could to substantially improved. The enrichment factors obtained under optimized conditions ranged from 2725 to 3879. The proposed method for detecting R,S-Mirtazapine and its metabolite provided strong linearity that ranged from 1.5 to 1500.0 nM, the limits of detection (LODs) ranged from 0.3 to 0.5 nM. In the real sample, the recovery of analytes for the urine sample at spiking levels of 1.5-150.0 nM and the serum sample at spiking levels of 5.0 – 250.0 nM were in the ranges of 89.0 – 116.5 % and 88.5 – 112.8 %, respectively. With the advantages of high enrichment factors and high accuracy and precision, the developed method was successfully applied to determine drugs in urine and serum.
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