| Summary: | 碩士 === 國立宜蘭大學 === 園藝學系碩士班 === 107 === Inoculation of 3 Tuber species from different origins (Tuber panzhihuanense from Panzhihuan City, and T. indicum and T. formosanum from Gaoligong Mountain and Huize County) to Quercus glauca were carried out in this study. There were 5 experimental groups and 1 control group. After 9 months of inoculation, ectomycorrhizae formed were identified by molecular and morphological identification, for accessing their affinity with Q. glauca host. Results showed that Q. glauca associated and formed ectomycorrhizae successfully with Tuber sp. in all experimental groups. Simple and monopodial – pinnate ectomycorrhizae were commonly observed in all groups. Monopodial-pyramidal ectomycorrhizae were rarely observed and only found on T. indicum group. Mantle of ectomycorrhizae formed by T. formosanum were wooly, stringy or smooth. Mantle formed by T. indicum were wooly or smooth while those formed by T. panzhihuanense were short-spiny. Results of molecular identification and phylogenetic analysis revealed that except groups of T. panzhihuanense and T. formosanum and T. indicum originated from Gaoligong Mountain, the groups of T. formosanum and T. indicum originated from Huize County formed ectomycorrhizae which were genetically not identical to the respective inoculums. The possible reasons might be the dispersal of spores by insects or small animals to other inoculated seedlings, or misplacement of inoculated seedlings to the wrong group. As a result, it is hard to compare the actual inoculation rates of the 5 experimental groups. Estimated association rate of T. indicum ectomycorrhizae was 39 %, T. formosanum ectomycorrhizae was 13 %, T. panzhihuanense ectomycorrhizae was 9 %, while rate of non-Tuber associated ectomycorrhizae was 39 %.
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