| Summary: | 碩士 === 國立宜蘭大學 === 生物技術與動物科學系動物科學碩士班 === 107 === The study is designed to test the effects of supplementing a multi-nutrient mixture of amino acids, omega-3 fatty acid, mineral and vitamins formulated to improve boar’s fertility that always depressed by heat stress. A total of 48 boars （Duroc, averaging 23 months）were randomly assigned to positive Control: offering 100 g starch per day per boar in addition to their normal commercial diet feeding（3.0 ± 0.2 kg）; Treatment: 100 g multi-nutrient formula instead of starch. Ejaculate was collected once or twice per week and its quality characteristics including semen volume and motility parameters were evaluated by CASA within 12 hours after each collection. The study lasted for 12 weeks, only samples on day 4th, 8th and 12th week were measured. During the experiment, the maximum and the minimum daily temperature was 34.2℃ and 23.5 ℃, with 88.6 % RH. The data were analyzed by Mixed model to reveal significant effects of the treatment. Results showed that a significant improvement（P < 0.01） was found in the normal morphology in the treatment group. Moreover, the parameters of motility indicated by CASA including VCL, VAP, VSL, and WOB were significant higher（P < 0.05）in the treatment group. It is also significantly increased the antioxidant capacity , protein concentration and aspartate-amino-transferase activity in the seminal plasma（P < 0.05）in the treatment group. The activity of alkaline phosphatase and γ-glutamyl-transferase reduce in seminal plasma（P < 0.05）in the treatment group. It is concluded that supplementation of prepared multi-nutrient formula to heat-stressed boars could improve their normal morphology and motility of sperm and suggesting that, under the subtropical summer, the nutrients supply to boars is good for them to exhibit their genetic potential in spermatogenesis in the current feeding regime.
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