| Summary: | 博士 === 國防醫學院 === 醫學科學研究所 === 107 === The chronic wounds healing is one of the major medical issues and wound care for patients with different diseases and the clinical significance of platelet-derived growth factor-AA (PDGF-AA) in the enhanced wound healing with stem cells and the precise molecular mechanism remains unclear. The major role of PDGF-AA in adipose-derived stem cells (ASCs) and endothelial progenitor cells (EPCs) enhancing wound healing was investigated in the present study. In this study, we applied the ASCs and EPCs to treat wounds in animal wound model by wound-healing assay. By using PDGF-AA short hairpin RNA or small hairpin RNA (shRNA) technique, PDGF-AA expression in ASCs was knocked down and the related molecular mechanism was investigated. Transplantation of ASCs could enhance wound healing from the results of the wound model and wound-healing assay. We found that PDGF-AA knock-down ASCs group had much less improvement of wound healing than other groups treated with wild-type ASCs in wound tissues. Through the PI3K/Akt/eNOS signaling pathway, the regulation of PDGF-AA in ASCs may contribute to improve wound healing. In the present study, we found that PDGF-AA might play a vital role in ASCs and EPCs enhancing wound healing. It would be a potential approach using PDGF-AA for clinical treatment of chronic wounds, possibly by its effects on angiogenesis.
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