| Summary: | 碩士 === 國立嘉義大學 === 微生物免疫與生物藥學系研究所 === 107 === Dendritic cells (DCs) are professional antigen-presenting cells. They are the key to initiate immune responses and induce immune tolerance. The generated tolerogenic DCs might be a useful tool for the induction of specific unresponsiveness. Luteolin is a kind of flavonoid that exists in many types of plants including vegetables, fruits, and medicinal herbs. It has multiple biological activities like anti-cancer and anti-oxidation. Besides, luteolin has been evidenced its anti-inflammatory effect through blockade of NF-κB activation in macrophages. Whether luteolin treated DCs can differentiate to suppressive status is still unknown. In this study, we investigated the influence of luteolin on the physiological characteristics and functions of DCs, and evaluate their ability to modulate T cell responses. Mouse DCs were matured with lipopolysaccharide (LPS) and tumor necrosis factor alpha (TNF-α). Allogeneic T cells, isolated from spleens of BALB/c mice, were co-cultured with luteolin-treated DCs, and then the proliferation of T cells was assessed. After treatment with luteolin, the expression of surface molecules (MHC-II, CD80, CD86, CD83, CD40, CD54 and CD274) of DCs was determined by FACS analysis. Besides, the level of mRNA in luteolin-treated DCs was also measured. The results showed that luteolin-treated DCs could strongly inhibit allogenic T-cell proliferation in a dose-dependent manner. Compared with non-treated DCs, the treatment of luteolin clearly decreased the expression of CD80 and CD86 molecules on DCs. Furthermore, the cytokine mRNA of IL-12p40, IL-10 and STAT-3 in DCs were inhibited by luteolin, and the cytokine mRNA of TGF-β was increased. These results suggested that luteolin changed the DCs to suppressive properties. It might be a tool to use in transplantation or DC-associated disease.
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