Effect of Metabolites of Antagonistic Microbes on the Control of Rice Sheath Blight

• Main Authors: LAI SU YU, 賴素玉
• Other Authors: Chen, Ruey-Shyang
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/r4d5m4

碩士 === 國立嘉義大學 === 生化科技學系研究所 === 107 === Rice sheath blight is one of the important rice diseases in Taiwan, and is mainly controlled by chemical agents. Antagonistic microorganisms which produce antimicrobial metabolites are widely used for biological control of plant diseases. In this study, the metabolites from Bacillus amyloliquefaciens BACY-1 and Trichoderma virens FT-333 were tested for their antagonistic activities against Rhizoctonia solani and were evaluated for the control of rice sheath blight. Using one-week cultured B. amyloliquefaciens filtrate (BCF), 100% of growth inhibition rate against Rhizoctonia solani were obtained, indicating the metabolite could significantly inhibit the mycelial growth and sclerotial germination of Rhizoctonia solani. While treated at 40°C, 60°C, 80°C and 121°C for 30 min, BCF still had good inhibitory activity against rice sheath blight. Using two-week cultured T. virens filtrate (TCF), 78% and 95.4% of growth inhibition rate on mycelial growth and sclerotial germination of Rhizoctonia solani were also observed. The inhibitory rates of B. amyloliquefaciens BCF on rice sheath blight were 89.2-92.2% by in vitro inoculation. In the greenhouse experiment, it was found that rice plants inoculated with Rhizoctonia solani after BCF pretreatment were not infected seriously. Based on the results of this study, BCF showed a better control effect as compared to TCF. The component and mechanism will be further studied in the futher. Keywords: rice sheath blight, Trichoderma virens, Bacillus amyloliquefaciens, metabolites, biological control