Could expression of the vitellogenin mRNA be the only biomarker to detect endocrine disrupting effect of plasticizers ?

• Main Author: 黃介廷
• Other Authors: 吳淑美
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/3vcfg8

碩士 === 國立嘉義大學 === 水生生物科學系研究所 === 107 === The vtg and ERs mRNA expression and E2 content of liver in male tilapia acted as biomarkers to detect whether seven plasticizers are endocrine disrupt chemicals (EDCs).The emission standard of these seven plasticizers were investigated in the present study in order to determine whether the seven common plasticizers induces any risks to the environment. Full male tilapia (Oreochromis. nilotica) was exposed to the concentrations in the range of emission standards published by Taiwan Environmental Protection Administration (TEPA) which are 0, 0.2, 0.4, and 0.8 mg/L of diethyl phthalate (DEP), dibutyl phthalate (DBP), butyl benzyl phthalate (BBP), diisononyl phthalate (DINP) and diisobutyl phthalate (DIBP); concentrations of 0, 0.1, 0.2, and 0.4 mg/L of Di-(2-ethylhexyl) phthalate (DEHP) ; and concentrations of 0, 0.4, 0.6, and 0.8 mg/L of di-n-octyl phthalate (DNOP) in the present study. The total RNA of liver was extracted from body after exposure to 7 plasticizers for 5 days, and vtg and ERs mRNA expressions of liver were analyzed by QPCR, and E2 content was determined by ELISA. Any one of the three factors (E2, ERs and vtg) shows a significant difference among treatments could be determined as risk inducer of EDC. The results showed that DEHP, DEP and DNOP appeared to be risk of EDC. In addition, although DINP and DIBP emission are not regulated by TEPA, either significant inhibition or increase on vtg mRNA expression of fish were shown after exposure to 0.2 and 0.4 mg/L of DINP and DIBP. There are no significant differences observed in other groups such as DBP and BBP as compare to the control group. In conclusion, we suggested that it is necessary to modify the concentration of emission standards of DEP, DNOP and DEHP for environmental safety.