| Summary: | 碩士 === 國立中央大學 === 生物醫學工程研究所 === 107 === The issue of heavy metal poisoning is very much concerned as it cause major risks for human health. Although copper (Cu) an essential element in the human body, excessive intake can have adverse effects on the systems in the human body. Poly amino acid was evaluated for possible use as an chelating agent in the treatment of metal intoxication in human body. Poly(serinyl acrylate) (PserA) were developed for its chelation with copper(II) ions. However, the precipitation formed will increase the toxicity level. Herein, we employ 2-methacryloyloxyethyl phosphorylcholine (MPC) to avoid precipitation and form metal colloids to decrease toxicity. We report a robust apporach to synthesize the double hydrophilic block copolymers(DHBCs) of PserA and PMPC via reversible addition-fragmentation chain transfer polymerization (RAFT). A variety of techniques were applied to confirm the synthesis and structure of this copolymer including 1H nuclear magnetic resonance (NMR), 13C NMR, fourier-transform infrared spectroscopy (FTIR), mass spectra (MS) and gel permeation chromatography (GPC). In addition, the chelating PMPC-b-PserA loaded to copper ions at pH = 7.2 were indicated by ultraviolet–visible spectroscopy(UV-vis). Dynamic light scattering (DLS) was applied to correlate between the Cu2+/serA ratio and the size of metal colloids at pH=7.2. We also studied influence of degree of polymerization(DP), pH effect, ionic strength and serum. Transmission electron microscopy (TEM) was also operated to take metal colloid's image. Finally, the double hydrophilic block copolymers(DHBCs) was tested on NIH-3T3 fibroblasts normal cells to study the detoxification effect. The double hydrophilic block copolymers possesses good biocompatibility to remove excess copper(II) ions and increase the cell viability in vitro. Hemolysis assay demonstrates that copolymer drastically decreases red blood cell(RBC) hemolysis from copper(II) in vitro. This strategy provides another treatment approach for heavy metal poisoning.
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